Journal: Frontiers in Psychiatry

Article Title: IgG/IgM-coated gut microbiota in schizophrenia: associations with inflammation disease activity

doi: 10.3389/fpsyt.2025.1689069

Figure Lengend Snippet: SCZ-associated IgG+/IgM+ bacteria induced colon pathology in C56BL/6J mice. (A) Colon length following gavage with SCZ-associated IgG+/IgM+ bacteria. ****p < 0.0001 (unpaired Student’s t-test). (B) Representative pathology and hematoxylin and eosin (H&E) stained colons from mice gavaged with SCZ-associated IgG+/IgM+ bacteria. Representative histological images of mice receiving IgG+/IgM+ exhibited extensive inflammation, crypt abscesses, epithelial loss, and ulceration, whereas mice receiving IgG-/IgM-induced mice showed no signs of Inflammation. Histopathological scores were assigned as follows: 0, intact colonic architecture with no acute inflammation or epithelial injury; 1, focal minimal acute inflammation; 2, focal mild acute inflammation; 3, severe acute inflammation with multiple crypt abscesses and/or focal ulceration; and 4, severe acute inflammation with multiple crypt abscesses, epithelial loss, and extensive ulceration. *p < 0.05 (unpaired Student’s t-test). (C) Percentage of neutrophil granulocytes in peripheral blood following induction of colitis by IgG+/IgM+ bacteria in C56BL/6J Mice. Ns (P>0.05); * (P<0.05); ** (P<0.01).

Article Snippet: For antibody staining, bacterial suspensions were blocked with staining buffer containing 20% normal mouse serum (Jackson ImmunoResearch, USA) for 20 min on ice, and incubated with 2% phycoerythrin(PE)-conjugated anti-human IgG/IgM antibodies (Miltenyi Biotec Germany) in staining buffer for 30 min on ice in the dark.

Techniques: Bacteria, Staining